Open AccessEVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS
Author(s) -
Biresh Kumar Sarkar,
Rahul Kumar,
Reeta,
Subash Chandra Verma,
Shashi Pal,
Ramaiah Maddi,
C. Murali Krishna,
Ravindra Singh
Publication year - 2017
Publication title -
international journal of current pharmaceutical research
Language(s) - English
Resource type - Journals
ISSN - 0975-7066
DOI - 10.22159/ijcpr.2017v9i5.22328
Subject(s) - anti inflammatory , in vitro , chemistry , lipoxygenase , cyclooxygenase , traditional medicine , phyllanthus , chromatography , biochemistry , enzyme , pharmacology , biology , medicine
Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.Â