Open AccessISOLATION OF ANTIBACTERIAL PROTEIN/PEPTIDE FROM FICUS GLOMERATA LEAF
Author(s) -
Manisha Thapliyal,
Anjali Bisht,
Ajeet Singh
Publication year - 2016
Publication title -
international journal of current pharmaceutical research
Language(s) - English
Resource type - Journals
ISSN - 0975-7066
DOI - 10.22159/ijcpr.2016v8i4.15271
Subject(s) - bacillus subtilis , antibacterial activity , pseudomonas aeruginosa , biology , microbiology and biotechnology , escherichia coli , bacteria , salmonella , pseudomonas , pathogen , ficus , botany , biochemistry , genetics , gene
Objective: To isolate the antibacterial proteins/peptides from Ficus glomerata leaf.Methods: Present study was designed to investigate antibacterial activity of proteins/peptides isolated from Ficus glomerata leaf. The isolated proteins/peptides were further checked for antibacterial activity against, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Salmonella entrica bacterial pathogens.Results: The results indicates that a 35kDa of protein were identified and exhibit good antibacterial activity against bacterial pathogen among all strains, Salmonella entrica and Pseudomonas aeruginosa exhibit good results with a clear zone of inhibition.Conclusion: Ficus glomerata is popular for its medicinal properties against therapeutic potential. In the present study a novel protein with broad spectrum antibacterial activity. Microbes cause severe damage to plants which results in a large economic loss so; this protein can be use as an active agent in agriculture for plant protection and also in the development of novel therapeutic agents.