Open AccessPHYTONUTRIENT SCREENING BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY AND ANTI-AGING PROPERTIES OF GLYCOSMIS PENTAPHYLLA (RETZ.) DC. (SOM-CHUEN) EXTRACTS
Author(s) -
Thisakorn Dumrongphuttidecha,
Warachate Khobjai,
Surachai Techaeoi,
Khemjira Jarmkom,
Suradwadee Thungmungmee
Publication year - 2021
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2021.v13s1.y0105
Subject(s) - stigmasterol , chemistry , bark (sound) , campesterol , gas chromatography–mass spectrometry , chromatography , phytol , traditional medicine , abts , food science , antioxidant , mass spectrometry , biochemistry , sterol , biology , dpph , cholesterol , medicine , ecology
Objective: The aims of this study were to screen the phytonutrient constituents and investigate their anti-aging property of leaves and bark ofGlycosmis pentaphylla (Retz.) DC. (GP).Methods: GP is the medicinal plant which used as traditional medicine. Glycosmis pentaphylla leaves (GPL) and Glycosmis pentaphylla bark (GPB) ofGP were extracted with ethanol. The gas chromatography–mass spectrometry was used for phytonutrients analysis. The anti-aging properties wereperformed using ABTS assay, deoxyribose degradation assay, bovine serum albumin (BSA)-fructose model, and matrix metalloproteinase 1 (MMP-1)inhibitory activity.Results: The important phytonutrients of GPL were α-tocopherol, linolenic acid, squalene, stigmasterol, and β-amyrin and for GPB were α-tocopherol,phytol, campesterol, stigmasterol, dictamine, and γ-sitosterol. The percentage inhibition of GPL and GPB extracts at various concentrations wasbetween 16.57–76.05 and 20.66-78.81 by ABTS assay, 68.24–90.06 and 73.83–96.64% by deoxyribose degradation assay, 4.24–99.98 and 54.81–99.94 by BSA-fructose model, and 6.31–81.55 and 1.06–74.45 by MMP-1 inhibitory activity, respectively.Conclusion: Leaves and bark extracts of GP (Retz.) DC. are good sources of important phytonutrients and have the potential in anti-aging property.