Open AccessBORON-SILICA BASED MESOPOROUS FOR CURCUMINOID ISOLATION FROM TURMERIC EXTRACT
Author(s) -
Adang Firmansyah,
Ilma Nugrahani,
Komar Ruslan Wirasutisna,
Slamet Ibrahim
Publication year - 2020
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2020v12i5.37041
Subject(s) - curcuminoid , mesoporous material , chemistry , adsorption , mesoporous silica , fourier transform infrared spectroscopy , ethyl acetate , elution , scanning electron microscope , extraction (chemistry) , chromatography , nuclear chemistry , solvent , materials science , chemical engineering , organic chemistry , catalysis , curcumin , biochemistry , engineering , composite material
Objective: The purpose of this study was to develop the isolation method for curcuminoid from turmeric extract using boron-silica based mesoporous as an adsorbent.
Methods: The formation of mesoporous materials were conducted using the sol-gel technique. The characterization of mesoporous materials was analyzed using a scanning electron microscope (SEM), transmission electron microscope (TEM), and Fourier transforms infrared spectrometry (FTIR). The extraction of turmeric was done by solvent extraction using ethanol 96 %. The isolation of curcuminoid was achieved by the adsorption method using mesoporous materials, both for silica-based mesoporous (MCM) and boron-silica based mesoporous (BMCM). The elution of curcuminoid-loaded mesoporous was conducted using various solvents. The concentration of total curcuminoid and its compounds was measured by visible spectrometry and high-performance liquid chromatography (HPLC).
Results: Morphology of MCM and BMCM shows the homogenous regular spherical shape, but having a different size. MCM has a smaller diameter particle size (500-600 nm) compared to BMCM (700-900 nm). On the other hand, BMCM has a smaller pore size (1-5 nm) compared to MCM (5-20 nm). The FTIR spectra of BMCM shows the additional vibration at 1400-1600 cm for B-O-H bond. Visible spectrometry measurement shows that the highest concentration of curcuminoid eluted from BMCM is 65.411±0.056 ppm by using ethyl acetate as a solvent, while the concentration of curcuminoid eluted from MCM is 11.503±0.054 ppm by using the same solvent. The results of curcuminoid adsorption and elution, indicating that ethyl acetate is the best solvent to elute curcuminoid due to its 98.83 % purity using HPLC analysis.
Conclusion: It was concluded that boron-silica based mesoporous showed stronger curcuminoid adsorption than silica-based mesoporous therefore found to be a potential adsorbent for curcuminoid isolation from turmeric extract.