Open AccessCOMPARISON OF ANTIFUNGAL EFFECT OF XANTHORRHIZOL (CURCUMA XANTHORRHIZA ROXB.) AND 2% CHLORHEXIDINE AGAINST CANDIDA ALBICANS AMERICAN TYPE CULTURE COLLECTION 10231 BIOFILM
Author(s) -
Sanny Tulim,
Kamizar Nazar,
Anggraini Margono,
Ratna Meidyawati,
Ermi Yanti
Publication year - 2020
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2020.v12s2.pp-04
Subject(s) - candida albicans , biofilm , corpus albicans , microbiology and biotechnology , curcuma , antifungal , chemistry , biology , traditional medicine , medicine , bacteria , genetics
Objective: Several studies suggest that 2% chlorhexidine (CHX), an effective irrigation solution against Candida albicans biofilm, is toxic to host cells,thus an effective and safe alternative irrigation solution is needed. Java turmeric (Curcuma xanthorrhiza Roxb.) containing xanthorrhizol (XNT) hasbeen reported to have an antifungal effect, yet no studies to date have reported the optimum dose of XNT in inhibiting C. albicans biofilm, so the aimof this study was to determine the optimum dose of XNT against C. albicans biofilm.Methods: C. albicans American Type and Culture Collection (ATCC) 10231 biofilm was exposed to XNT for 15 min. Then, the antifungal effect wastested using 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenytetrazolium bromide reduction assay and total plate count (TPC).Results: There was no statistically significant difference between the percentage of biofilm eradication and TPC results following exposure ofC. albicans ATCC 10231 biofilm to 1% XNT, 1.25% XNT, and 2% CHX.Conclusion: Our results suggest that 1% XNT and 1.25% XNT have an antifungal effect against C. albicans ATCC 10231 biofilm equivalent to that of2% CHX.