Open AccessINHIBITORY EFFECTS OF SANGKETAN (ACHYRANTHES ASPERA L.) ROOT EXTRACTS ON ARGINASE ACTIVITY
Author(s) -
Dieah Siti Rahmawati,
Berna Elya,
Arikadia Noviani
Publication year - 2020
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2020.v12s1.ff054
Subject(s) - arginase , chemistry , achyranthes aspera , ethyl acetate , flavonoid , traditional medicine , chromatography , extraction (chemistry) , potency , nitric oxide , hexane , arginine , biochemistry , in vitro , antioxidant , organic chemistry , medicine , alternative medicine , amino acid , pathology
Objective: Achyranthes aspera, commonly called Sangketan in Indonesian, is a wild plant used as traditional medicine. The roots of Sangketan can beused as a wound healer, which is attributed to the involvement of arginine and its metabolites, nitric oxide, which directly influence the wound healingprocess. The aim of the present study was to determine the potential of Sangketan root extracts to inhibit arginase activity.Methods: The roots were extracted using the multistage ultrasound-assisted extraction method using n-hexane, ethyl acetate, and methanol solvents.Each of the extract associated with the three solvents was tested for arginase inhibition activity using a microplate-based colorimetric method,followed by the determination of total phenolic compound and total flavonoid concentrations.Results: The results of the inhibition test of arginase activity based on n-hexane, ethyl acetate, and methanolic extracts were 9.56, 17.58, and 29.77%,respectively, at concentrations of 100 μg/mL; the total phenolic compound concentrations were 3.91, 4.83, and 11.18 mg GAE/g of sample, respectively,and the total flavonoid concentrations were 0.29, 0.80, and 0.88 mg QE/g of sample, respectively.Conclusion: According to the results, Sangketan root extracts had low potency with regard to arginase inhibitory activity.