Open AccessRESPONSE SURFACE METHODOLOGY APPLIED TO OXALIC ACID HYDROLYSIS OF OIL PALM EMPTY FRUIT BUNCH BIOMASS FOR D-XYLOSE PRODUCTION
Author(s) -
Herman Suryad,
Arry Yanuar,
Harmita,
Putri Winny Rachmadani
Publication year - 2020
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2020.v12s1.ff037
Subject(s) - xylose , oxalic acid , hemicellulose , hydrolysate , chemistry , hydrolysis , furfural , central composite design , chromatography , response surface methodology , acid hydrolysis , sulfuric acid , yield (engineering) , nuclear chemistry , organic chemistry , materials science , fermentation , catalysis , metallurgy
Objective: The study aimed to identify the best conditions using oxalic acid for hydrolysis of hemicellulose in oil palm empty fruit bunch (OPEFB)biomass.Methods: The analytical method of high-performance liquid chromatography (HPLC) was using a SUPELCOSIL LC-NH2 column, refractive indexdetection detector, and three compositions of the mobile phase. At first, the hydrolysis of hemicellulose in OPEFB powder was optimized by applyinga response surface methodology. A three-variable, six-central composite design was used for the experiments. Temperature (between 95°C and135°C), reaction time (between 10 and 110 min), and oxalic acid concentration (between 1% and 7% [w/v]) were evaluated by running 15 differentexperiments at constant biomass concentrations. Then, hydrolysis was optimized again at the constant temperature selected with three variables:OPEFB concentration, reaction time, and oxalic acid concentration. Hydrolysate samples were detoxified with carbon active, and furfural compoundwas analyzed by gas chromatography with flame ionization detector.Results: The optimum condition of HPLC was using acetonitrile: water (9:1) at a flow rate of 1.0 ml/min. The first hydrolysis results showeda high yield of D-xylose produced, which was 6.40 g D-xylose/100 g OPEFB biomass, with a xylose recovery of 93.8%. However, this result wasnot yet optimum. Further hydrolysis at constant temperature experiment produced the highest xylose yield of 13.13%, equivalent to 32 g/lD-xylose.Conclusion: The yield of D-xylose from mild hydrolysis using oxalic acid was similar to that using dilute sulfuric acid as used in the previous studyby Rahman et al.