Open AccessLIPOXYGENASE INHIBITORY ASSAY OF ETHYL ACETATE FRACTION FROM STAR FRUIT LEAVES (AVERRHOA CARAMBOLA L.) FROM THREE REGIONS IN WEST JAVA
Author(s) -
Defi Suciana Subardini,
Berna Elya,
Arikadia Noviani
Publication year - 2020
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2020.v12s1.ff025
Subject(s) - averrhoa carambola , chemistry , ethyl acetate , flavonoid , gallic acid , chromatography , phenol , ic50 , maceration (sewage) , phenols , lipoxygenase , food science , enzyme , biochemistry , antioxidant , in vitro , organic chemistry , materials science , composite material
Objective: Averrhoa carambola L. leaves have been used as a traditional medicine. The study aimed to examine the anti-inflammatory activity ofA. carambola L. plant extracts using a lipoxygenase inhibition assay based on ethyl acetate fractions of A. carambola L. leaves obtained from threeregions in West Java.Methods: Extracts from A. carambola L. leaves were obtained by maceration method using 70% ethanol followed by fractionation using liquid-liquidpartitioning until the ethyl acetate fraction was obtained. Subsequently, lipoxygenase inhibition activity of the ethyl acetate fraction was tested usinglinoleate acid as the substrate, and the total flavonoid and total phenol contents were determined. The correlation between the IC50 values and thetotal flavonoid and total phenol contents obtained from the ethyl acetate fraction were analyzed.Results: The ethyl acetate fraction from the sample inhibited lipoxygenase enzyme activity with IC50 values of 19.38, 16.65, and 15.07 μg/mL, respectively.The total flavonoid contents in the ethyl acetate fractions obtained from Depok, Subang, and Sukabumi regions were 14.88, 16.88, and 22.27 mg QE/gof sample, respectively, whereas the total phenol contents were 54.10, 61.06, and 72.18 mg gallic acid equivalents/g of sample, respectively.Conclusion: There was a high correlation between the IC50 values and the total flavonoid and total phenol contents, with correlations coefficientsof −0.917 and −0.960, respectively, which indicated that the higher the total flavonoid and total phenol contents, the lower the IC50 values.