Open AccessOSTEOCALCIN GENE POLYMORPHISM IN INDONESIAN MEN WITH PERIODONTITIS
Author(s) -
Namira Kaulika,
Talitha Alwasris,
Niniarti Djamal,
Benso Sulijaya,
Christopher J. Talbot,
Elza Ibrahim Auerkari
Publication year - 2020
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2020.v12s1.arl028
Subject(s) - genotype , periodontitis , polymorphism (computer science) , medicine , agarose gel electrophoresis , aggressive periodontitis , gene polymorphism , allele , osteocalcin , polymerase chain reaction , genotyping , restriction fragment length polymorphism , gastroenterology , biology , genetics , gene , enzyme , biochemistry , alkaline phosphatase
Objective: Through this work, we aimed to clarify the association between the severity of periodontitis and osteocalcin (OC) gene C298T polymorphismin Indonesian male subjects.Methods: DNA was extracted from blood serum samples of 100 consenting Indonesian men whose periodontitis was classified as mild, moderate,or severe. Polymerase chain reaction and restriction fragment length polymorphism techniques were applied to evaluate the status of OC (C298T)polymorphism using HindIII restriction enzyme and electrophoresis in agarose gel to separate the indicated fragments.Results: Although genotype distribution of the OC C298T polymorphism showed a trend for more frequent occurrence of genotype hh (and allele h)with increasing severity of periodontitis, this trend was not statistically significant in the tested sample population, in whom the HH genotypeappeared to be relatively rare.Conclusion: The results showed no significant association between the severity of periodontitis and OC C298T polymorphism in Indonesian malesubjects.