Open AccessASSOCIATION BETWEEN MATRIX METALLOPROTEINASE-1 −1607 1G/2G POLYMORPHISM AND CHRONIC PERIODONTITIS IN INDONESIAN POPULATION
Author(s) -
Rahmatul Hayati,
Antonius Winoto Suhartono,
Sri Lelyati C. Masulili,
Christopher J. Talbot,
Elza Ibrahim Auerkari
Publication year - 2020
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2020.v12s1.arl026
Subject(s) - chronic periodontitis , genotype , periodontitis , allele , gastroenterology , polymorphism (computer science) , medicine , matrix metalloproteinase 9 , matrix metalloproteinase , genomic dna , polymerase chain reaction , genetics , immunology , biology , gene
Objective: This study aimed to identify the distribution of matrix metalloproteinase (MMP)-1 −1607 1G/2G alleles and genotypes in subjects withchronic periodontitis and healthy subjects in a sample of Indonesian population and assess the possible association of this polymorphism withsusceptibility to chronic periodontitis.Methods: Genomic DNA samples were obtained from 200 Indonesian males aged 33–78 years old, comprising 100 chronic periodontitis patientsand 100 healthy controls. DNA fragments were amplified by a polymerase chain reaction and analyzed by restriction fragment length polymorphism.Results were analyzed by Chi-square test.Results: The frequency of the 2G allele was high both in subjects with periodontitis (87%) and in controls (91%). Analysis of MMP-1 genotype(−1607 1G/2G) showed no significant difference between the chronic periodontitis and healthy groups (p>0.05).Conclusion: The result found no association between MMP-1 −1607 1G/2G polymorphism and susceptibility to chronic periodontitis in Indonesiansubjects.