Open AccessEFFECT OF DAYAK ONION TUBER (ELEUTHERINE AMERICANA MERR.) EXTRACT TO PREVENT INCREASED NECROSIS OF KIDNEY TUBULAR EPITHELIAL CELLS IN MICE (MUS MUSCULUS) TO ORAL LEAD ACETATE EXPOSURE
Author(s) -
Gadis Meinar Sari,
Daeng Agus Vieya Putri,
Tjitra Wardani,
Lilik Herawati
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s5.t1016
Subject(s) - lead acetate , necrosis , karyorrhexis , kidney , staining , biology , medicine , toxicity , biochemistry , endocrinology , programmed cell death , apoptosis , genetics
Objective: This study aims to determine the effect of Dayak onion tuber extract to prevent an increase in necrosis of kidney proximal tubular epithelialcells exposed to lead acetate.Methods: Fresh Dayak onion tuber was extracted with 96% ethanol and then given orally to mice with doses of 65 mg/kg, 130 mg/kg, and 260 mg/kgfor 21 days, accompanied by 14 days of lead acetate on the 8th–21st days. Histological preparations of the mice’s kidney were made by hematoxylinand eosin staining, and then, the necrosis of tubular cells was examined using a ×400 magnification light microscope that observed 5 views randomlymarked by the presence of pyknosis, karyorrhexis, and karyolysis.Results: This study showed the higher the dose of Dayak onion tuber extract and the lower the number of tubular epithelial cell necrosis (p<0.05).Conclusion: Extract of Dayak onion tuber could prevent an increase in necrosis of kidney proximal tubular cells of mice that exposed to lead acetate.