Open AccessEVALUATION OF ACETYLCHOLINESTERASE ACTIVITY AND CYTOTOXICITY OF DIFFERENT PARTS OF NELUMBO NUCIFERA GAERTN ON HUMAN NEUROBLASTOMA CELL LINE (SH-SY5Y)
Author(s) -
Khemjira Jarmkom,
Surachai Techaoei,
Nakuntwalai Wisidsri,
Warachate Khobjai
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s5.t0080
Subject(s) - sh sy5y , acetylcholinesterase , aché , cytotoxicity , lotus , cell culture , acetylcholine , nelumbo nucifera , chemistry , ic50 , pharmacology , cholinergic , cholinergic neuron , cholinesterase , in vitro , traditional medicine , neuroblastoma , biochemistry , biology , enzyme , endocrinology , medicine , botany , genetics
Objective: Cognitive deterioration occurring in patients with probable Alzheimer’s disease is associated with a progressive loss of cholinergic neuronsand a consequent decline in levels of acetylcholine in the brain. This study aimed to evaluate the acetylcholinesterase (AChE) inhibitory effects andcytotoxicity in SH-SY5Y cells of different parts of three lotus extracts.Methods: AChE activity was quantified by spectrophotometry and cytotoxicity by flow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromideassay in SH-SY5Y cells exposed to extracts.Results: All of the extracts had inhibitory effects to AChE at p<0.05, but Roseum Plenum stem extract could inhibit AChE more than 30% (p<0.05). Theall of the extracts could be an increase SH-SY5Y cell proliferation, while Album Plenum flower extract could be cytotoxic on SH-SY5Y cells.Conclusion: The extracts of lotus could be supplemented compound for cognitive deterioration or Alzheimer’s patients.