Open AccessCYTOTOXIC ACTIVITY OF ERIOCAULON CINEREUM R.BR TO MCF-7 AND VERO CELL LINE
Author(s) -
Arde Toga Nugraha,
Asgar Purnama,
Siti Nurul Komariah,
HADY ANSHORI T,
HADY ANSHORI T
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s5.t0073
Subject(s) - ethyl acetate , dichloromethane , vero cell , ic50 , chemistry , chromatography , cytotoxic t cell , bromide , mcf 7 , nuclear chemistry , in vitro , biology , biochemistry , solvent , organic chemistry , cancer cell , human breast , cancer , genetics
Objective: The aim of the study was to determine the strength of the Eriocaulon cinereum R.Br plant against breast cancer cells (MCF7) and cytotoxicagainst Vero cells.Methods: E. cinereum R.Br was taken from the province of Bangka Belitung and then extracted in stages with n-hexane, ethyl acetate, and methanol.Then, the ethyl acetate extract was fractionated using the vacuum liquid chromatography method with dichloromethane and ethyl acetate solvents.Sample was tested for MCF-7 cells and Vero cells using the MTT method (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). The dataobtained is analyzed by probit SPSS.Results: The results of this study showed that the best IC50 extract was ethyl acetate extract with an IC50 value of 450.31 μg/ml. Then, ethyl acetateextract produced dichloromethane fraction with IC50 value of 443.52 μg/ml and ethyl acetate with IC50 value of 214.75 μg/ml. Ethyl acetate fractionwas also tested against Vero cells to see cytotoxic against normal human cells with IC50 679.11 μg/ml