Open AccessDETERMINATION OF LUPEOL FROM MAKHAEN AT THAILAND AND TAIWAN BY HIGHPERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
Surachai Techaoei,
Khemjira Jarmkom,
Nakuntwalai Wisidsri,
Suradwadee Thungmungmee,
Warachate Khobjai
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s5.t0045
Subject(s) - lupeol , chiang mai , chromatography , bark (sound) , methanol , high performance liquid chromatography , extraction (chemistry) , traditional medicine , chemistry , thin layer chromatography , column chromatography , solvent , toluene , biology , medicine , organic chemistry , ecology , ethnology , history
Objective: The objective of this research was to investigate the extraction and isolation of chemical compounds of Zanthoxylum limonella Alstonextracts which selected from Chiang Mai and Pa Yao province, Thailand and Taiwan.Method: There are 3 locations of medicinal plant, Z. limonella Alston was collected, two provinces of northern Thailand, Chiang Mai and Phayao,including the other country, Taiwan. All of extractions were carried out by methanol. In this experimental study, thin-layer chromatography (TLC) andhigh-performance liquid chromatography (HPLC) were measured.Results: The result was found that the best organic solvent for extraction is methanol. The suitable of extraction was methanol and toluene in the ratioof 9:1 when developed with TLC and gradient methanol and water when analyzed with HPLC. The potential bioactive compound from crude extractedwas lupeol. Crude extracted from leaf at Chiang Mai was highest lupeol as 5655.72 μg/mg extraction whereas crude extracted from leaf at Phayaoshowed this compound as 16, 98.81 μg/mg extraction. In addition, the crude extraction from root and bark of both areas showed the amount of lupeolless than leaf crude extracted. The further study will focus on antimicrobial activity and biological activity in the near future.Conclusion: The bioactive compound, lupeol was found in all part of Makhaen in Thailand but did not found in Taiwan seed.