Open AccessGARLIC EXTRACT EFFICACY AGAINST THE VIABILITY OF ENTEROCOCCUS FAECALIS (IN VITRO)
Author(s) -
Andriani Octavia,
Sarworini Bagio Budiardjo,
Ike Siti Indiarti,
Eva Fauziah,
Margaretha Suharsini,
Heriandi Sutadi,
Mochamad Fahlevi Rizal
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s1.17351
Subject(s) - enterococcus faecalis , chlorhexidine , microbiology and biotechnology , enterococcus , food science , gram positive bacteria , chemistry , traditional medicine , bacteria , biology , staphylococcus aureus , medicine , antimicrobial , antibiotics , dentistry , genetics
Objective: Enterococcus faecalis, Gram-positive facultative anaerobe capable of invading the dentinal tubules, is resistant to several irrigating solutionsand intracanal medications. Motivated by the antibacterial effect exhibited by garlic extract against a wide variety of Gram-positive and Gram-negativebacteria, as well as anaerobic bacteria such as Lactobacillus and E. faecalis, we aimed in this study to analyze the efficacy of garlic extract against theviability of E. faecalis from clinical isolates of nonvital primary root canals.Methods: We used MTT assay to determine the viability of E. faecalis after exposure to increasing concentrations of garlic extract (10%, 25%, 50%,and 100%) and chlorhexidine (CHX) 2% as a positive control.Results: We performed the analysis using the Kruskal–Wallis test and the post hoc test for multiple comparisons at a 0.05 significance level. At allconcentrations, garlic extracts decreased the viability of E. faecalis. We found no significant differences between the viability values for 25%, 50%,and 100% garlic extracts (p>0.05); however, they were significantly different from the 10% extract and CHX 2% (p<0.05), both with a lower viabilityvalues.Conclusion: Our results showed that garlic extract was effective in decreasing the viability of E. faecalis.