Open AccessDIFFERENCES IN THE POTENTIAL MUTAGENICITY OF RESIN-, SILICONE-, AND BIOCERAMIC-BASED SEALERS ON LYMPHOCYTES: A PROTEIN EXPRESSION ANALYSIS
Author(s) -
Sani Suryadarma,
Kamizar,
Endang Suprastiwi,
Ratna Meidyawati,
Maida Fitri
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s1.16021
Subject(s) - bioceramic , silicone , dentistry , significant difference , chemistry , chromatography , materials science , medicine , composite material
Objective: The objective of this study was to compare the potential mutagenicity of resin-, silicone-, and bioceramic-based sealers on proteinexpression in human lymphocytes. There has been limited research on resin-, silicone-, and bioceramic-based sealers effects on protein expressionin lymphocytes.Methods: Nine samples of each sealer were incubated in 2 mL human blood for 1, 3, and 7 days. Then, the isolated lymphocytes are observed forprotein separation by electrophoresis method. Profile of protein bands observed and data were analyzed statistically by Kruskal–Wallis and post hocMann–Whitney.Results: Although no statistically significant differences in protein bands were observed among the resin-, silicone-, and bioceramic-based sealers(p=0.111), there was a statistically significant difference between the resin- and silicone-based sealers on the 1st day (p=0.046) and 3rd day (p=0.046)and between the silicone- and bioceramic-based sealers on the 1st day (p=0.046). Thus, the present study shows that there were differences in thepotential mutagenicity on the 1st day; resin was potentially more mutagenic followed by bioceramic and silicone. On the 3rd and 7th days, bioceramicwas potentially more mutagenic followed by resin and silicone.Conclusion: The manuscript describes the study in detail and concludes that resin was potentially more mutagenic followed by bioceramic- andsilicone-based sealers.