Open AccessPOTENTIAL OF JAVANESE TURMERIC ETHANOL EXTRACT IN INHIBITING STREPTOCOCCUS SANGUINIS AND PORPHYROMONAS GINGIVALIS BIOFILM FORMATION
Author(s) -
Reina Hutauruk,
Dewi Fatma Suniarti,
Widurini Djohan
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s1.154
Subject(s) - streptococcus sanguinis , biofilm , porphyromonas gingivalis , microbiology and biotechnology , chemistry , agar , ic50 , staining , bacteria , in vitro , biology , biochemistry , genetics
Objective: The purpose of the current study was to evaluate the potential of Javanese turmeric ethanol extract in the inhibition of the formation ofStreptococcus sanguinis and Porphyromonas gingivalis biofilms, individually and in combination.Methods: The concentration of P. gingivalis and S. sanguinis bacteria ranged from 0.5% to 25%. Inhibition assay for biofilm formation was conductedon a 96-well plate using brain heart infusion (BHI) agar enriched with 0.2% sucrose at 37oC for 18 h. After staining with 0.5% crystal violet, the opticaldensity was measured at 490 nm.Results: Javanese turmeric can potentially inhibit the biofilm formation of S. sanguinis (IC50 0.5%, IC90 15%) and P. gingivalis (IC50 15%) on single anddual species (IC50 0.5%, IC90 15%).Conclusion: Javanese turmeric has the potential to inhibit the formation of S. sanguinis and P. gingivalis biofilms.