ACCELERATED WOUND HEALING ABILITY OF SACRAN HYDROGEL FILM BY KERATINOCYTE GROWTH FACTOR IN ALLOXAN-INDUCED DIABETIC MICE | Zendy

Nasrul Wathoni | Zendy; Aliya Nur Hasanah | Zendy; Ahmed Fouad Abdelwahab Mohammed | Zendy; Elasari Dwi Pratiwi | Zendy; Ripa’atul Mahmudah | Zendy

Open Access

ACCELERATED WOUND HEALING ABILITY OF SACRAN HYDROGEL FILM BY KERATINOCYTE GROWTH FACTOR IN ALLOXAN-INDUCED DIABETIC MICE

Author(s) -

Nasrul Wathoni,

Aliya Nur Hasanah,

Ahmed Fouad Abdelwahab Mohammed,

Elasari Dwi Pratiwi,

Ripa’atul Mahmudah

Publication year - 2018

Publication title -

international journal of applied pharmaceutics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.238

H-Index - 15

ISSN - 0975-7058

DOI - 10.22159/ijap.2018v10i2.24217

Subject(s) - keratinocyte growth factor , ultimate tensile strength , swelling , wound healing , materials science , hepatocyte growth factor , biomedical engineering , andrology , chemistry , growth factor , composite material , medicine , biochemistry , surgery , receptor

Objective: The main objective of the research work was to fabricate sacran hydrogel film containing keratinocyte growth factor (Sacran/KGF-HGF), and to evaluate their wound healing ability in alloxan-induced diabetic mice model.Methods: The physicochemical characterization of Sacran/KGF-HGF were investigated by thickness, tensile strength, swelling ratio, x-ray diffractometer (XRD), scanning electron microscope (SEM), and biodegradability. The wound healing ability was investigated by creating two full-thickness excisional wounds inalloxan-induced diabetic mice.Results: The thickness, tensile strength, and swelling ratio results showed that KGF in the Sacran/KGF-HGF improved not only the thickness of sacran hydrogel film (Sacran-HGF), but also the tensile strength and swelling ability of Sacran-HGF. The XRD and SEM results confirmed that the Sacran/KGF-HGF were amorphous and similar morphology to Sacran-HGF, respectively. The biodegradability results revealed that the Sacran/KGF-HGF degraded for about 41.29% in trichloroacetic acid (TCA) and 22.92% in TrypLE™ (recombinant enzyme) solutions. In addition, KGF improved the degradability of Sacran/KGF-HGF in both solutions. Interestingly, the Sacran/KGF-HGF, which was applied on wound site, considerably improved the wound healing ability of Sacran-HGF at 6, 9 and 12 d in alloxan-induced diabetic mice model, compared to control (non-treated).Conclusion: These results suggest that KGF has the potential to promote the chronic wound healing ability of Sacran-HGF.

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