Open AccessA NOVEL OF BEZAFIBRATE ANALYSIS METHODS IN URINE (IN VITRO) USING SOLID PHASE EXTRACTION– HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-UV DETECTOR
Author(s) -
Iyan Sopyan,
Widiastuty Irawati,
Wiwiek Indriyati
Publication year - 2018
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2018v10i1.22769
Subject(s) - chromatography , bezafibrate , detection limit , solid phase extraction , extraction (chemistry) , high performance liquid chromatography , chemistry , urine , cartridge , materials science , biochemistry , metallurgy
Objective: Bezafibrate is the second generation of fibrate groups used as the drug of choice in the treatment of hyperlipidemia. The purpose of this study is to obtained a validated method for analyzing bezafibrate in urine using solid phase extraction (SPE)-High performance liquid chromatography (HPLC).Methods: Solid phase extraction (SPE) using hydrophilic-lipophilic balance (HLB) cartridge was performed for bezafibrate extraction from urine, afterward, a validation of analysis method using high-performance liquid chromatography (HPLC)-(UV) detection was conducted to parameters, including: selectivity (Rs), linearity (r), accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). Results: Recovery extraction using SPE resulted %recovery 85-110%. The analysis was performed by high-performance liquid chromatography using reversed phase, C18 octadecylsilane (ODS) columns 250 x 26 mm, particle size 10 μl, with the composition of 0.01 M acetate buffer with pH 3.55: with percent composition (45:55) and 0.8 ml/minute on 230 nm UV detection. Validation includes selectivity, linearity, accuracy, precision LOD, and LOQ have fulfilled requirement value. Conclusion: The result of recovery extraction using SPE and validation of method exhibited the values that fulfilled the requirements and can be used for analysis bezafibrate in the urine.