Open AccessEFFICACY OF IONIC LIQUID [MIM]BR-BASED MAE ON RESVERATROL AND PHENOLIC COMPOUNDS EXTRACTION FROM GNETUM GNEMON SEEDS AND THEIR DPP-4 INHIBITORY ACTIVITY
Author(s) -
Arif Arrahman,
Rezi Riadhi Syahdi,
Hana Permatasari,
Intan Fikri Purnama Sari,
Abdul Mun’im
Publication year - 2018
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2018.v10s1.36
Subject(s) - extraction (chemistry) , ionic liquid , chemistry , gallic acid , chromatography , bromide , resveratrol , solvent , organic chemistry , catalysis , biochemistry , antioxidant
Objective: This study aimed to determine the inhibitory activity of 1-butyl-3-methylimidazolium bromide ([bmim]Br) extracts of melinjo seeds(Gnetum gnemon) on dipeptidyl peptidase-4 (DPP-4).Methods: Melinjo seeds were extracted by a [bmim]Br microwave-assisted method using various extraction parameters and the inhibitory activityof DPP-4 of all extracts was determined in 96-well microplates using Cayman inhibitor screening assay. Determination of trans-resveratrol contentwas conducted using a reverse-phase high-performance liquid chromatography method. The total phenolic content was determined using a 96-wellmicroplate reader. Data analysis for the determination of the optimal extraction conditions was developed by response surface methodology.Results: The extract obtained from the third run showed the highest inhibition (28.73%) against DPP-4 activity with the total phenolic content of1.96 mg gallic acid equivalent/g the seed powder.Conclusion: The analytical results revealed the following optimal conditions: Solvent concentration 1.5 M, liquid-solid ratio 23:1, and extraction time15 min.