Open AccessCYCLEA BARBATA LEAF EXTRACT: LIPOXYGENASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING
Author(s) -
Nadya Febri Handayani,
Berna Elya,
Nuraini Puspitasari
Publication year - 2018
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2018.v10s1.22
Subject(s) - chemistry , phytochemical , flavonoid , ethyl acetate , ic50 , lipoxygenase , glycoside , arachidonate 5 lipoxygenase , linoleic acid , terpenoid , chromatography , traditional medicine , antioxidant , food science , biochemistry , enzyme , in vitro , stereochemistry , fatty acid , arachidonic acid , medicine
Objective: The aim of this study was to test the anti-inflammatory activity of methanol, ethyl acetate, and n-hexane Cyclea barbata Mier. leaf extractsusing a lipoxygenase inhibition method.Methods: Enzyme activity assay was performed using colorimetric method with lipoxygenase from soybean and linoleic acid as a substrate.Absorbance was measured at 234 nm. The total flavonoid content of the most active extract was determined using a colorimetric method with AlCl3and phytochemical screening.Results: The ethyl acetate extract had the highest lipoxygenase inhibiting activity, with an IC50 value of 0.267 mg/mL. In addition, this extract had atotal flavonoid content of 21.62 mgQE/g and contained flavonoids, glycosides, and terpenoids.Conclusion: This finding indicated that C. barbata provides a possible anti-inflammatory effect through the inhibition of lipoxygenase. However,further research is still needed to strengthen this result.