Open AccessANALYSIS OF ALPHA-LINOLENIC ACID AND DOCOSAHEXAENOIC ACID IN MACKEREL FISH OIL (RASTRELLIGER KANAGURTA) USING GAS CHROMATOGRAPHY
Author(s) -
Tafdlilul Arfan,
Harmita,
Baitha Palanggatan Maggadani
Publication year - 2018
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2018.v10s1.07
Subject(s) - docosahexaenoic acid , chromatography , fish oil , chemistry , mackerel , derivatization , gas chromatography , toluene , fatty acid , fish <actinopterygii> , high performance liquid chromatography , polyunsaturated fatty acid , fishery , biochemistry , biology , organic chemistry
Objective: This study aimed to obtain the levels of alpha-linolenic acid (ALA) and docosahexaenoic acid (DHA) in mackerel fish oil by pressing andextraction with solvent methods.Methods: The optimum conditions were determined, and validation methods were performed for a mixture of ALA and DHA to obtain a valid methodfor the determination of the levels of ALA and DHA in mackerel fish oil. Derivatization was performed by the Lepage esterification method usingmethanol:toluene 4:1 (v/v) and an acetyl chloride catalyst. Gas chromatography with the Shimadzu GC-17A with a DB-5 column and flame ionizationdetector was used to analyze samples at a column temperature of 200°C with an increase of 2°C/min up to 230°C (maintained for 20 min). Injectorand detector temperatures of 250°C were used with a flow rate of 1.00 mL/min.Results: The retention time of ALA and DHA was 11.440 min and 22.337 min with a Tf of 0.949 and 1.006, respectively. The validation results fulfilledthe acceptance criteria with r values of 0.99953 and 0.99934, respectively. Total levels of ALA and DHA in mackerel fish oil were 0.39521% by pressingand 0.33014% by extraction with solvents.Conclusion: This method could be used as an alternative method to analyze ALA and DHA level in fish oil.