Open AccessTHE ANTIBACTERIAL EFFECT OF A DIODE LASER USED AS AN ADJUNCT IRRIGANT ON CLINICAL ISOLATE OF ENTEROCOCCUS FAECALIS BIOFILM (IN VITRO)
Author(s) -
Ayu Sandini,
Ratna Meidyawati,
Kamizar,
Dewa Ayu Npa
Publication year - 2018
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2017.v9s2.25
Subject(s) - enterococcus faecalis , sodium hypochlorite , chlorhexidine , biofilm , hypochlorite , microbiology and biotechnology , saline , chemistry , bacteria , dentistry , medicine , biology , staphylococcus aureus , organic chemistry , genetics
Objective: The elimination of bacteria from the root canal has always been a problem in root canal management, and Enterococcus faecalis often found in the persistent intraradicular infections that occur after poor or unsuccessful endodontic treatments. The use of an irrigant with a diode laser adjunct eliminates this bacteria. To analyze and compare the effectiveness of a diode laser, chlorhexidine 2%, and natrium hypochlorite 2.5% on a clinical isolate of E. faecalis biofilms.Methods: Using E. faecalis biofilms from clinical isolate were grown on microtiter well plate, incubated for 24 h and subjected to the following treatments: Natrium hypochlorite 2.5% (5 s), chlorhexidine 2% (5 s), natrium chloride 0.9% (5 s), and the irrigants with additional diode laser irradiation (980 nm, 15 Hz, 1.5 W, 3.5 J, 5 s). The antibacterial effects of the irrigants and diode laser were scored using colony form units (CFU).Results: The clinical isolate colony of E. faecalis that was exposed to a saline solution and diode laser application had the highest score (18700 CFU/ml), while the lowest score (80,00 CFU/ml) was recorded in the group that was exposed to a chlorhexidine 2% irrigant with additional diode laser application.Conclusion: The diode laser had an antibacterial effect on a clinical isolate of E. faecalis biofilm, and this effect was increased when it was used in addition to the application of chlorhexidine 2% and natrium hypochlorite 2.5% irrigants.