Open AccessANALYTICAL VALIDATION OF CLOPIDOGREL IN HUMAN PLASMA THROUGH ULTRAHIGHPERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
Author(s) -
Yahdiana Harahap,
Innet Maysyarah
Publication year - 2017
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2017.v9s1.79_86
Subject(s) - chromatography , chemistry , formic acid , protein precipitation , electrospray ionization , acetonitrile , mass spectrometry , high performance liquid chromatography , tandem mass spectrometry , selected reaction monitoring , liquid chromatography–mass spectrometry , analytical chemistry (journal)
Objective: This study developed a sensitive, selective, and valid method for analyzing clopidogrel in human plasma using liquid chromatographytandemmass spectrometry (LC-MS/MS).Methods: The chromatography separation was performed on the waters ACQUITY UPLC Class BEH C18 1.7 μm (2.1 × 100 mm) column, consisting of0.1% formic acid in water and 0.1% formic acid in acetonitrile (30-70) as the mobile phase, and an isocratic elution with a flow rate of 0.2 mL/minutes.The mass detection was performed using a Waters Xevo TQD equipped with positive electrospray ionization in the multiple reaction monitoringmodes. The clopidogrel was detected at m/z 322.086>212.097 and irbesartan as an internal standard at m/z 429.233>207.131. The sample wasprepared with protein precipitation method using acetonitrile, vortex mixed for 10 minutes, and centrifuged at 13,000 rpm for 20 minutes.Results: This method showed a linear result at the concentration range of 0.2-10 ng/ml with r≥0.9997.Conclusions: The developed method provides sensitivity, linearity, precision, and accuracy and is suitable for analysis of clopidogrel in human plasmausing LC-MS/MS.