Open AccessDO INDONESIAN GOVERNMENT DEPLOY RELIABLE AMMUNITION FOR COVID-19 MASS TEST? A COMPARISON OF REAL-TIME PCR KITS
Author(s) -
Nelly Marissa,
Salmiaty,
Sari Hanum,
Evan Febriansyah,
Nur Ghaniaviyanto Ramadhan,
Yulidar,
Zain Hadifah,
Agung Pranata
Publication year - 2021
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2021.v14i5.40078
Subject(s) - covid-19 , real time polymerase chain reaction , virology , coronavirus , medicine , pcr test , polymerase chain reaction , biology , gene , disease , pathology , infectious disease (medical specialty) , genetics
Objective: This study aimed to compare the level of reliability of three commercial real-time PCR kits in determining clinical samples.
Methods: A total of 40 swabs samples which were previously tested positive, were re-test using the BioCov-19 RT-PCR kit, Sansure coronavirus disease 19 (COVID-19) nucleic acid diagnostic kit, and Kogen PowerCheck with Thermocycler (Roche). The amplification procedure is carried out based on the manual for each kit.
Results: Sansure COVID-19 nucleic acid diagnostic was able to detect 40 samples with positive severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) results detected in both genes, while the PowerChek™ 2019-nCoV real-time PCR kit able to detect 35 samples showed that SARS-COV-II was detected in both genes, and the BioCoV-19 RT-PCR Kit brand kit able to detect 34 samples showed positive SARS-COV-2 results in both genes.
Conclusion: The three commercial kits show great ability detection, so that they can be used to detect the presence of SARS-COV-II in clinical samples, and also in mass screening.