Open AccessIN VIVO EFFICACY OF TOPICAL BINAHONG (ANREDERA CORDIFOLIA (TEN.) STEENIS) LEAF ETHANOLIC EXTRACT ON TRANSFORMING GROWTH FACTOR-BETA 1 IN INFECTED WOUNDS
Author(s) -
Rizki Andini Nawawi,
Muhammad Totong Kamaluddin,
- Theodorus
Publication year - 2021
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2021.v14i3.40516
Subject(s) - medicine , traditional medicine , in vivo , staphylococcus aureus , antibiotics , biology , bacteria , microbiology and biotechnology , genetics
Objective: This study’s aim was to assess the efficacy of topical Binahong (Anredera cordifolia (Ten.) Steenis) leaf ethanolic extract administration on serum transforming growth factor-beta 1 (TGF-β1) in infected wounds.
Methods: An experimental study, in vivo, was conducted in the Biotechnology Laboratory and Animal House, Faculty of Medicine, Universitas Sriwijaya, Palembang, from July to September 2020. There were 30 male Wistar rats aged 10–12 weeks with excisional wounds infected with Staphylococcus aureus ATCC 25923. The rats were divided into five groups and received three concentrations of Binahong leaf extracts (2.5%, 5%, and 10%), salve base, and povidone iodine 10% topically twice daily for 14 days. Serum was obtained before treatment and after 14 days of treatment. Wound area and bacterial count were also recorded and analyzed. Data analysis was performed using computer software.
Results: Wound size and bacterial count were significantly decreased in treatment groups receiving topical Binahong leaf ethanolic extract. No significant increase in serum TGF-β1 was observed in all treatment groups.
Conclusion: Topical administration of Binahong leaf ethanolic extract on rats with infected wounds for 14 days did not significantly increase serum TGF-β1.