Open AccessTOXICOLOGICAL AND BEHAVIOR ANALYSES OF A FOLIOSE LICHEN PARMOTREMA TINCTORUM (DELISE EX NYL.) HALE EXTRACT USING ZEBRAFISH (DANIO RERIO) MODEL
Author(s) -
Muthu Shenbagam,
Moorthiraman Murugan,
Kalidoss Rajendran,
Machampalayam Arumugam Deepa,
P. Ponmurugan
Publication year - 2021
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2021.v14i10.42134
Subject(s) - zebrafish , danio , in vivo , ex vivo , apoptosis , lichen , biology , toxicity , chemistry , andrology , botany , biochemistry , gene , medicine , genetics , organic chemistry
Objective: The objective of the study was to evaluate the toxicity and behavior analysis with the extract of foliose lichen Parmotrema tinctorum against the adult zebrafish.Methods: The zebrafishes were exposed to different concentrations of P. tinctorum extract such as 50, 100, and 200 mg/ml for 7 days of exposure, in which the mortality and behavioral responses of zebrafishes were recorded. The standard histopathological examination was conducted with the lichen extracts of P. tinctorum.Results: The results revealed that P. tinctorum extract did not show any prominent behavior abnormalities in zebrafishes even at a high concentration of 200 mg/ml. The extract was found to have dose-dependent toxic to zebrafish and the number of neutrophil cells in the muscle bundles reduced at a high concentration. The results of the inflammatory marker gene expression using polymerase chain reaction results suggested that the dose-dependent suppression of tumor necrosis factor gene by the P. tinctorum lichen extract.Conclusion: Overall, concluded that the extract might contain anti-inflammatory induction properties and further tests are required to prove apoptosis and anticancer activity using other in vivo or in vitro techniques.