GAS CHROMATOGRAPHY AND MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS OF DRYOPTERIS HIRTIPES (BLUMZE) KUNTZE

Objective: The objective of this study was to analyze GC–MS analysis of whole plant methanolic extract of Dryopteris hirtipes from Dryopteridaceae family. Methods: Gas chromatography and mass spectrometry analysis of whole plant extract was carried out with instrument GC–MS. Results: The methanolic extract of D. hirtipes reveals to identify more known and unknown bioactive compounds. In this study, seven major bioactive compounds were identified such as Stigmast-5-en-3-ol(56.65%), Phytol (5.39%), Lanost -8-en-3-ol-(3 β)(3.18%), Neophytadiene(2.68%), Tri-o-trimethylsilyl N-heptaflurobutryl derivative of terbutaline(2.19%), 1H-Imidazole 2-methanol(1.28%), and 8A-(2,4-Dimethyl-1-nitrilo-pent-2-yl) dioxy)tocopherone(1.0%) and low concentrations of compounds like hexadecanoic acid(0.6%). Conclusion: These identified compounds are having active pharmacological properties such as antimicrobial property, hypotension, anti-inflammatory, anti-tumor, anti-cancer, anti-hepatitis, analgesic, and antipyretic properties. However, D. hirtipes is a rare pteridophyte and used to cure many diseases, and so there need further studies to isolate and identify the specific active compounds present in it.