Open AccessBACTERIAL DNA EXTRACTION METHOD FOR THE DETECTION OF HELICOBACTER PYLORI FROM HUMAN FECAL SAMPLES
Author(s) -
Jhoan Fabricio Guaman,
Favian Bayas-Morejón,
Elena Brito,
Norma Paredes
Publication year - 2020
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2020.v13i6.37344
Subject(s) - polymerase chain reaction , dna extraction , helicobacter pylori , extraction (chemistry) , feces , helicobacter , dna , pathogen , chromatography , microbiology and biotechnology , biology , chemistry , gene , genetics
Objective: The work proposed the implementation of a method of DNA extraction for the detection of the pathogen from 50 stool samples.
Methods: A method of DNA extraction with Chelex resin was applied and then detected by conventional polymerase chain reaction (PCR).
Results: By PCR, 11 samples were positive for Helicobacter pylori.
Conclusions: The Chelex extraction methodology allows obtaining DNA with quality necessary to be detected by PCR, making it a fast methodology for its diagnostic application.