Open AccessACHATINA FULICA MUCUS ATTENUATES ULTRAVIOLET B-INDUCED FIBROBLAST PHOTOAGING THROUGH REDUCING INFLAMMATION, ANGIOGENESIS, AND MATRIX METALLOPROTEINASE
Author(s) -
Ch. Tri Nuryana,
Sofia Miceli,
Yohanes Widodo Wirohadidjojo,
Nur Arfian,
PUTU MEGA ADITYA DEVI AYU MARA,
AHMAD FAIZ HUWAIDI
Publication year - 2020
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2020.v13i5.37284
Subject(s) - photoaging , matrix metalloproteinase , angiogenesis , fibroblast , inflammation , chemistry , dermal fibroblast , wound healing , vascular endothelial growth factor , matrix (chemical analysis) , microbiology and biotechnology , immunology , vegf receptors , cancer research , medicine , biochemistry , biology , dermatology , chromatography , in vitro
Objective: This study aimed to observe the effects of Achatina fulica mucus (AFM) on ultraviolet B (UVB)-induced fibroblast photoaging by assessing monocyte chemotactic protein (MCP)-1, vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMP)-3, and MMP-12 mRNA expressions.
Methods: Cell cultures of normal human dermal fibroblasts (NHDFs) were divided into six groups: Group 1 was normal fibroblasts without UVB irradiation as normal control and Groups 2–5 consisted of 100 mJ/cm2 UVB-induced aged fibroblasts. Group 2 had no treatment as negative control, Group 3 was treated by platelet-rich plasma 10% as positive control group, and Groups 4–6 were treated by various concentrations of AFM (3.9 μl, 15.625 μl, and 62.5 μl). The MCP-1, VEGF, MMP-3, and MMP-12 mRNA expressions in the different NHDF groups were assessed by quantitative polymerase chain reaction.
Results: The mRNA expressions of MCP-1, VEGF, MMP-3, and MMP-12 in the AFM group compared to the UVB group decreased 8, 5, 5, and 4 folds, respectively. AF62 exhibited the highest improvement among the other AFM-treated groups.
Conclusion: AFM treatment attenuates UVB-induced fibroblasts photoaging by reducing inflammation, angiogenesis, and matrix metalloproteinases.