Open AccessIN VITRO EVALUATION OF ENZYMIC ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITY IN THE ETHANOLIC EXTRACT OF CRESCENTIA CUJETE LEAVES
Author(s) -
P ANITHA,
NAZEEMA TH
Publication year - 2020
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2020.v13i4.36794
Subject(s) - chemistry , ascorbic acid , antioxidant , glutathione , dpph , glutathione reductase , superoxide dismutase , catalase , glutathione peroxidase , hydroxyl radical , nitric oxide , biochemistry , enzyme , food science , organic chemistry
Objectives: This study aims to evaluate the enzymic antioxidants and free radical scavenging present in the ethanolic leaf extracts of Crescentia cujete.
Methods: Enzymic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase, glutathione peroxidase, and glutathione S-transferase (GST) were estimated by standard methods. Free radical scavenging potential was evaluated by diphenylpicrylhydrazyl (DPPH), nitric oxide, and hydroxyl radical methods using an ethanolic extract of C. cujete leaf.
Results: The leaf extract of C. cujete showed the maximum activity of CAT, SOD, GST, glutathione reductase, and peroxidase activity. CAT activity was formed to be highest in the ethanolic extract of C. cujete leaf. DPPH radical scavenging activity was reported as 38.5 μg/ml, nitric oxide was found to be 200.77 μg/ml, and hydroxyl radical scavenging exhibited 108.42 μg/ml normalized with ascorbic acid.
Conclusion: From the results, it has concluded that the ethanol extract of the C. cujete leaf has a prospective source of natural antioxidant that would be a great significance as therapeutic agents in preventing or slowing the progress of reactive oxygen species and related oxidative stress-related degenerative diseases.