Open AccessISOLATION OF ACTINOMYCETES FROM THE SEDIMENTS OF PICHAVARAM MANGROVE FOREST, SOUTH INDIA AND ANALYSING THEIR ANTIBACTERIAL EFFICACY
Author(s) -
M AARTHI,
D KAMALANATHAN,
V BALAKRISHNAN
Publication year - 2020
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2020.v13i4.36702
Subject(s) - mangrove , antibacterial activity , agar , isolation (microbiology) , biology , bacteria , nutrient agar , ethyl acetate , agar plate , microbiology and biotechnology , food science , chemistry , ecology , biochemistry , genetics
Objective: The aim of the present investigation is to isolate actinomycetes from the sediments of Pichavaram mangrove forest, South India, and to screen for their antibacterial efficiency.
Methods: Actinomycetes were isolated by culturing the samples in Starch Casein Agar medium; they were screened primarily for their antibacterial efficiency against Gram-positive and Gram-negative bacterial organisms. Solvent extraction was done with 50% (percentage) ethyl acetate, crude extracts of actinomycetes were prepared at different concentrations using dimethyl sulfoxide and treated against the bacterial organisms. Antibacterial assay was done in Mueller–Hinton agar medium.
Results: Thirteen actinomycetes were isolated; among them, four actinomycete isolates (Pichavaram mangrove actinomycete 2 [PMA2], PMA6, PMA9, and PMA13) exhibited antibacterial activity.
Conclusion: Isolate PMA2 exhibited very strong antibacterial activity and isolate PMA13 is weakly active against the tested bacterial organisms.