Open AccessEVALUATION OF THE EFFECT OF PIPER BETLE L. LEAVES EXTRACT AGAINST CLONIDINE-INDUCED CATALEPSY AND MILK-INDUCED LEUKOCYTOSIS AND EOSINOPHILIA IN MICE
Author(s) -
Ramdas N. Kale,
Ravindra Patil
Publication year - 2020
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2020.v13i11.39321
Subject(s) - leukocytosis , catalepsy , clonidine , eosinophilia , piper , pharmacology , medicine , traditional medicine , chemistry , anesthesia , immunology , haloperidol , dopamine
Objective: The objective of the study was to evaluate the effect of Piper betle L. leaves extract against clonidine-induced catalepsy and milk-induced leukocytosis and eosinophilia in mice.
Methods: Methanolic extract of P. betle L. leaves was prepared using Soxhlet apparatus. Preliminary phytochemical screening of the prepared extract was carried out using standard chemical tests. Effect of the prepared extract was evaluated against clonidine-induced catalepsy and milk-induced leukocytosis and eosinophilia in mice model.
Results: Maximum duration of catalepsy was observed at 90 min after the clonidine administration. There was a significant inhibition (p˂0.05) of clonidine-induced catalepsy in the animals pretreated with chlorpheniramine maleate and extract of P. betle leaves. Administration of milk (4 mg/kg) subcutaneous route exhibited a significant increase in leucocytes and eosinophil count after 24 h of administration. Methanolic extract of P. betle L. leaves showed significant inhibition (p˂0.05) of milk-induced leukocytosis and eosinophilia.
Conclusion: These results suggest that P. betle leaves extract may have the potential therapeutic value in the treatment of allergic diseases.