Open AccessEFFECT OF STERCULIA QUADRIFIDA R. BR BARK TO IN VITRO DPPH RADICAL AND GLUTATHIONE PEROXIDASE ON DIABETIC WISTAR RATS
Author(s) -
Rambu Konda Anggung Praing,
Titik Sunarni
Publication year - 2020
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2020.v13i11.38805
Subject(s) - dpph , chemistry , antioxidant , bark (sound) , glutathione peroxidase , ethanol , traditional medicine , biochemistry , food science , medicine , catalase , biology , ecology
Objective: The aims of the research were to evaluate the antioxidant effect of Sterculia quadrifida R.Br bark by in vitro 1,1-biphenyl-2-picryl-hydrazyl (DPPH) radical scavenging method and glutathione peroxidase (GPx) activity on diabetic Wistar rats model.
Methods: S. quadrifida bark powder was extracted in ethanol 70% by maceration method. The antioxidant activity of ethanol extract was tested by DPPH radical scavenging method. The activity of GPx was evaluated from the liver of alloxan-induced diabetic rats. The antioxidant activity was determined by measuring the absorbance using ultraviolet-visible spectrophotometry method.
Results: The ethanol extract of S. quadrifida bark had a strong DPPH radical scavenging activity, with the IC50 value was 4.86±0.01 ug/ml, besides that the extract also showed significantly activity of GPx (p<0.05) at a dose of 65, 130, and 260 mg/kg bw.
Conclusion: The ethanol extract of S. quadrifida bark had a potent antioxidant activity by DPPH radical scavenging and GPx activity.