Open AccessIN VITRO EVALUATION OF AEGLE MARMELOS LEAF EXTRACTS ON FOOT ULCER AND URINARY TRACT INFECTED PATHOGENS FROM DIABETIC PATIENTS
Author(s) -
Mujawar Salma Begum Hussain,
Murigendra B Hiremath
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2020.v13i1.36514
Subject(s) - urinary system , urine , diabetic foot ulcer , antimicrobial , thin layer chromatography , agar , in vitro , ethanol , antibacterial activity , diabetes mellitus , traditional medicine , medicinal plants , column chromatography , nutrient agar , medicine , chemistry , diabetic foot , chromatography , microbiology and biotechnology , biology , bacteria , biochemistry , genetics , endocrinology
Objective: The objective of the study was to evaluate the effect of leaf extracts on foot ulcer and urinary tract pathogens from patients having diabetes.
Methods: The pus and urine sample from the patients having diabetes were collected and cultured on nutrient agar plates. The morphological characters were studied for the colonies obtained. Antimicrobial activity of ethanol and aqueous extracts was studied for Aegle marmelos leaves.
Results: Ethanol extract exhibited good results for both foot ulcer and urinary tract infected pathogens as compared to aqueous extract. Thin-layer chromatography was performed for methanol extract and then was subjected to column chromatography. The fractions obtained were again checked for the antibacterial activity and of three fractions, fraction 1 showed better results for pus and urine pathogens. Fourier-transformed infrared analysis was carried out for fraction 1 of methanol extract for verifying the presence of bioactive compounds.
Conclusion: From the present study, it can be terminated that the bioactive compounds derived from plant extracts can be used for the development of new pharmaceutical drugs to reach out to various therapeutic needs.