Open AccessTHE GREEN SYNTHESIS OF SILVER NANOPARTICLES OF ONION DNA AND SCREENING FOR IN VITRO ANTITYROSINASE ACTIVITY
Author(s) -
Thakur Sneha,
Krishna Mohan G
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i9.34745
Subject(s) - fourier transform infrared spectroscopy , dynamic light scattering , nuclear chemistry , silver nanoparticle , chemistry , scanning electron microscope , silver nitrate , dna , particle size , nanoparticle , crystallography , materials science , analytical chemistry (journal) , nanotechnology , chemical engineering , chromatography , biochemistry , engineering , composite material
Objective: The main objective of the research work is to evaluate the antityrosinase potential of onion DNA silver nanoparticles (AgNPs).
Methods: The onions were procured from the local market and DNA was extracted from onions using detergent and methylated spirit. The isolated DNA was selected for synthesis of AgNPs which acts as capping and reducing agent. About 10 ml of the DNA extract was added to 90 ml of 0.1 N silver nitrate solution. After 24 h incubation, the solution turned dark brown, which indicates the formation of AgNPs. The synthesized DNA AgNPs were characterized by ultraviolet-visible, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) studies.
Results: The results revealed that the particles were uniform in shape with face-centered cubic structure. The particles are 153±20.4 nm in size and were no signs of agglomeration measured by DLS studies. The FTIR spectroscopy revealed B form of DNA along with strong N-H stretching, C=N stretching, and also asymmetric vibrations of phosphate groups characteristic for DNA molecule. The XRD studies revealed the face-centered cubic structure. SEM studies revealed the spherical structure with average particle size of 150±0.1 nm for single DNA nanoparticles. The onion DNA AgNPs were further investigated for its antityrosinase activity against the standard kojic acid and were to have anticancer potential nearer to the standard.
Conclusion: From the results, it is evident that the synthesized onion DNA AgNPs have antityrosinase potential and can be further investigated for in vivo anticancer potential in future.