Open AccessCOMPARATIVE PHYTOCHEMICAL PROFILE AND ANTIOXIDANT PROPERTY OF BARK, FLOWERS AND LEAVES EXTRACTS OF SIMAROUBA GLAUCA
Author(s) -
N Sajeeda,
Kolgi Rr,
Shivakumara Sl,
S. Yallappa,
Karigar Cs
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i9.34211
Subject(s) - phytochemical , antioxidant , chemistry , bark (sound) , ascorbic acid , gallic acid , traditional medicine , flavonoid , extraction (chemistry) , chloroform , food science , botany , biology , chromatography , biochemistry , medicine , ecology
Objective: The current study was to evaluate and compare the phytochemical constituents and antioxidant activity of bark, flowers and leaves of the tree Simarouba glauca.
Methods: The solvent extraction of phytochemicals was carried out using Soxhlet apparatus with ethanol, chloroform, methanol, and water. The antioxidant property was determined by 2,2-Diphenyl-1-picrylhydrazyl, hydrogen peroxide free radical scavenging, reducing power assay, and nitric oxide radical scavenging assay using gallic acid and ascorbic acid as the standards.
Results: The extraction yield was found maximum in the water extract of flower (3.7% w/w). Qualitative and quantitative analysis of phytoconstituents showed that the highest amount of alkaloids and flavonoid content (2.1% w/w) and (3.9% w/w), respectively, was in the chloroform extract of the flower. Phenol and carbohydrate constituent was found to be highest in the methanol extract of leaves 2.5% w/w and 2.2% w/w, respectively. The antioxidant assays showed that the bark possessed maximum antioxidant activity. The water extracts of S. glauca bark exhibited scavenging property (90%) with an IC50 value 39.63 μg/ml, and the least activity (56%) was observed in the methanol extracts of leaves with an IC50 value of 62.96 μg/ml of S. glauca.
Conclusion: The study concluded that the water extract of the bark is a potent antioxidant compared to leaves and flowers. Further, in vivo studies are essential to enumerate its medicinal use and prove its efficacy in therapeutic applications.