Open AccessANTIMICROBIAL ACTIVITIES OF SUCCESSIVE SOLVENT EXTRACTS OF ALBIZIA LEBBECK AND SOLANUM SEAFORTHIANUM AGAINST SOME HUMAN PATHOGENIC MICROORGANISMS
Author(s) -
Hosur Narayanappa Venkatesh,
Devihalli Chikkaiah Mohana
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i6.32706
Subject(s) - antimicrobial , petroleum ether , minimum inhibitory concentration , pathogenic bacteria , chemistry , bacteria , antibacterial activity , agar diffusion test , preservative , yeast , chloroform , pseudomonas aeruginosa , microbiology and biotechnology , traditional medicine , food science , chromatography , biology , extraction (chemistry) , biochemistry , medicine , genetics
Objective: The continuous emergence of multidrug resistance bacteria and yeast, and the negative impact of synthetic preservatives have led to an increased interest in the use of plant extracts as alternative antimicrobial agents. In the present investigation, the antimicrobial activity of successive solvent extracts of Albizia lebbeck and Solanum seaforthianum has been evaluated against human pathogenic bacteria and yeast.
Methods: The disc diffusion method was employed for determination of the zone of inhibitions (ZOIs) and twofold broth dilution technique was employed for determination of minimal inhibitory concentrations, and minimal bactericidal/fungicidal concentrations.
Results: Among the successive solvent extracts tested, methanol extracts of both A. lebbeck and S. seaforthianum showed highest antibacterial activity with ZOIs ranged between 10.0 and 20.4 mm at 1 mg/disc followed by ethanol (ZOIs 8.1–17.6 mm). The petroleum ether, toluene, and chloroform extracts showed the least activity. The highest activity was observed against Streptococcus faecalis, whereas the least activity was observed against Pseudomonas aeruginosa.
Conclusion: The broad-spectrum antimicrobial activity of methanol extract of A. lebbeck and S. seaforthianum could be explored as antimicrobial agents for the management of pathogenic bacteria and yeast.