Open AccessIN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF THE FLOWER EXTRACTS OF OPUNTIA STRICTA
Author(s) -
Dorairaj Prabhakaran,
A. Rajeshkanna,
Senthamilselvi Mm
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i3.29930
Subject(s) - antioxidant , ethyl acetate , dpph , nitric oxide , anti inflammatory , chemistry , peripheral blood mononuclear cell , in vitro , ferric , viability assay , lipopolysaccharide , food science , traditional medicine , biochemistry , pharmacology , biology , organic chemistry , medicine , immunology
Objective: The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Opuntia stricta.
Methods: The flower extract was evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by ferric-reducing capacity (FRC) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method.
Results: The solid powder obtained from the ethyl acetate fraction from the flower O. stricta showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS-induced PBMCs) assay and NO assay.
Conclusion: These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower O. stricta has significant antioxidant and anti-inflammatory activities.