Open AccessNARINGENIN-LOADED D-Α-TOCOPHERYL POLYETHYLENE GLYCOL SUCCINATE 1000 POLYMERIC NANOSUSPENSION: AN IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITY
Author(s) -
Sumathi Rajamani,
Gobinath Kalyana Sundaram,
Tamizharasi Sengodan,
Sivakumar Thangavelu,
Nikhitha K Shanmukhan,
Arun Radhakrishnan
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i2.29331
Subject(s) - in vivo , naringenin , chemistry , bioavailability , pharmacology , diclofenac , in vitro , carrageenan , polyethylene glycol , flavonoid , chromatography , biochemistry , antioxidant , medicine , biology , microbiology and biotechnology
Objective: Naringenin (NAR) a flavonoid, exhibits extensive pharmacological action, fails to attain a significance in application due to low aqueous solubility (~ 0.214 mg/mL) which results in low bioavailability (5.8%). Nanosuspension of NAR (NARNS) was prepared in our previous studies using high-pressure homogenization employing various polymers. All these formulations were characterized and as a continuation of our work formulations was further evaluated for their anti-inflammatory activity by in vitro and in vivo methods.
Methods: Denaturation of protein method and membrane stabilization methods was chosen for in vitro evaluation. In vivo studies performed were acute inflammatory studies (carrageenan-induced paw edema) and chronic inflammatory studies (cotton pellet granuloma) on Wistar albino rats.
Results: The studies demonstrated that the NAR and NARNS at a dose of 50mg/kg P.O. have a potent activity compared to the standard drug diclofenac.
Conclusion: The percentage of protection against inflammation exhibited by NARNS was highly significant compared to NAR.