Open AccessINSULIN SECRETAGOGUE EFFECT OF ROOTS OF RAVENALA MADAGASCARIENSIS SONN. - AN IN VITRO STUDY
Author(s) -
Sakthi Priyadarsini S,
PR Kumar,
Abhishek Anand,
B Devendiran,
Venkat S Kadiyam,
Richie Padmanabh Roy
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i2.29011
Subject(s) - secretagogue , insulin , insulinoma , viability assay , cytotoxicity , medicine , endocrinology , in vitro , chemistry , secretion , pharmacology , biology , biochemistry
Objective: The objective of this study was to establish the cytotoxicity profile and to evaluate the insulin secretagogue effect of ethanolic root extract of Ravenala madagascariensis Sonn.
Methods: The cell viability of rat insulinoma 5F (RIN5F) cell lines over the treatment of plant extract was assessed by 3-(4,5-dimethyl-2-thiazolyl)- 2,5-diphenyltetrazolium bromide assay. The insulin-releasing effect was evaluated by insulin secretion assay over RIN5F cell lines by enzyme-linked immunosorbent assay.
Results: The ethanolic extract of the roots of R. madagascariensis Sonn. showed negligible cytotoxicity at 20–40 μg/ml, and hence, concentrations up to 40 μg/ml were used in insulin secretion assay. The ethanolic root extract at 20 and 40 μg/ml significantly (p<0.05 compared to control) stimulated the insulin release in a dose-dependent manner even in the presence of glucose at lower and higher concentrations (5 and 10 mM).
Conclusion: Thus, our results validate its traditional claim in the treatment of diabetes by stimulating the secretion of insulin, thereby suggesting a possible mechanism of its antidiabetic effect.