Open AccessSPECTROPHOTOMETRIC DETERMINATION OF TOTAL PHENOLIC CONTENT FOR STANDARDIZATION OF VARIOUS PHYLLANTHUS SPECIES
Author(s) -
Rakhi Khabiya
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i18.34323
Subject(s) - phyllanthus , gallic acid , chemistry , phyllanthus emblica , traditional medicine , food science , antioxidant , organic chemistry , medicine
Objective: This study aims at comparison of total phenolic content of ethanolic extract of various species of Phyllanthus available in Gujarat state by spectrophotometric method.
Methods : The total phenolic content was determined quantitatively using the Folin Ciocalteu reagent, with Gallic acid as standard.
Results: The total phenolic content in methanolic extract of various Phyllanthus species ranged from 41.801 to 87.542 mg/g of dry weight of extract, expressed as gallic acid equivalents. Phyllanthus urinaria was found to have highest phenolic content and Phyllanthus acidus was found to have lowest phenolic content .
Conclusion: The total phenolic content will be helpful for developing new drugs and standardizing the various Phyllanthus species particularly morphological similar species. The presence of a high total phenolic content shows that the alcoholic extract of Phyllanthus may possess antioxidant properties, which could lead to a new field of research in the future.