Open AccessResearch Article LEAVES EXTRACT-BASED BIOGENIC SYNTHESIS OF CUPRIC OXIDE NANOPARTICLES, CHARACTERIZATIONS, AND ANTIMICROBIAL ACTIVITY
Author(s) -
Y. Prakash
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i18.34128
Subject(s) - distilled water , nuclear chemistry , antimicrobial , streptococcus mutans , antibacterial activity , chemistry , agar diffusion test , scanning electron microscope , escherichia coli , chromatography , materials science , bacteria , organic chemistry , biochemistry , biology , gene , composite material , genetics
Objectives: Tulsi (Ocimum tenuiflorum) leaves extract-based synthesis of cupric oxide nanoparticles (CuONPs), characterizations, and antimicrobial activity.
Methods: The small cut leaves were washed with double distilled water and boiled for 30 min. After filtration, the extract was treated with 0.2 M copper acetate solution and the initial color change of this solution indicated formation of copper nanoparticles. This solution was stirred for a specific time, heated and treated with 0.1 M NaOH solution. The formation of CuONPs was confirmed by the development of brownish-black precipitates. Then, CuONPs have been tested for their antibacterial effects by applying well diffusion method against Escherichia coli, Streptococcus mutans, Proteus vulgaris, and Staphylococcus aureus.
Results: The biologically synthesized CuONPs have been well characterized by using ultraviolet-visible, Fourier-transform infrared, X-ray powder diffraction, and field-emission scanning electron microscopy techniques and all these analytical methods indicated a successful and efficient formation of CuONPs. After the incubation period, significant zones of inhibition were observed for E. coli, S. mutans, P. vulgaris, and S. aureus.
Conclusions: The method was found highly efficient, eco-friendly, and low cost for the synthesis of biologically important CuONPs. The CuONPs have been found an excellent antibacterial agent.