Open AccessTERATOGENICITY TESTING OF SIDDHA FORMULATION OF NILAVEMBU KUDINEER ON ZEBRAFISH (DANIO RERIO) EMBRYO
Author(s) -
P Shanmugapriya,
S Elansekaran,
Muralidharan Thoddi Ramamurthy,
Jeeva Gladys R,
D. Sivaraman,
Christian GJ
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2019.v12i18.33664
Subject(s) - danio , embryo , hatching , zebrafish , human fertilization , andrology , biology , beagle , toxicology , anatomy , medicine , zoology , fishery , biochemistry , ecology , gene
Objective: The aim of the study was to evaluate the teratogenic effect of Nilavembu Kudineer (NVK) by testing in zebrafish embryo (Danio rerio).
Methods: The study consisted of 30 embryos/culture plate/dose concentration containing a series of diluted decoction of NVK ranging from 10 μg to 640 μg/ml, and the embryo development was monitored at specific time points. The parameters such as developmental abnormality and adverse events were monitored at 24 hourly intervals for 96 h.
Results: The study results showed 100% hatching and survival of embryos with no significant abnormalities in the extension of study from 96 to 120 h post-fertilization examinations.
Conclusion: NVK did not have teratogenic potential on testing it in various concentrations on zebrafish embryos validating its safety during pregnancy.