Open AccessETHANOLIC EXTRACT OF TITHONIA DIVERSIFOLIA (HEMSLEY) A. GRAY INHIBITS MIGRATION ACTIVITY AND DECREASE THE TRANSFORMING GROWTH FACTOR-BETA1, VEGF EXPRESSION ON KELOID FIBROBLASTS
Author(s) -
Mae Sri Hartati Wahyuningsih,
Dwi Aris Agung Nugrahaningsih,
Arief Budiyanto
Publication year - 2019
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v12i1.29850
Subject(s) - keloid , fibroblast , in vitro , vascular endothelial growth factor , transforming growth factor , wound healing , vegf receptors , chemistry , cancer research , microbiology and biotechnology , pharmacology , medicine , immunology , biology , pathology , biochemistry
Objectives: Keloid occurred due to abnormal wound healing, characterized by massive fibroblast proliferation and excessive collagen accumulation. Tithonia diversifolia Hemsley A. Gray has been known to show antiproliferative effect against some cancer cells in vitro. However, its potential as anti-keloid has not been explored. This study aims to assess the T. diversifolia ethanolic extract effect on fibroblast migration activity, transforming growth factor-beta1 (TGF-β1), and vascular endothelial growth factor (VEGF) expression of keloid fibroblasts in vitro.Methods: Fibroblasts were isolated from keloid collected from patient keloid tissue. The migration activity of keloid fibroblasts was assessed using scratch assay. The TGF-β1 and VEGF expression examination was done using ELISA.Results: Ethanolic extract of T. diversifolia treatment at a concentration of 20 μg/ml, 10 μg/ml, and 5 μg/mL for 24 h on keloid fibroblasts culture showed slower migration activity compare to those on keloid fibroblast culture without treatment (p<0.05). The TGF-β1 and VEGF expression was significantly lower in ethanolic extract of T. diversifolia treatment group compared to those on keloid fibroblast without treatment (p<0.05).Conclusion: Ethanolic extract of T. diversifolia inhibits fibroblast migration activity and decrease the expression of VEGF and TGF-β1 on keloid fibroblasts in vitro.