Open AccessANTIOXIDANT ACTIVITY OF ALKALOID FRACTIONS OF ZANTHOXYLUM ACANTHOPODIUM DC. FRUITS WITH 1,1-DIPHENYL-2-PICRYLHYDRAZYL ASSAY
Author(s) -
Rosidah Rosidah,
Poppy Anjelisa Zaitun Hasibuan,
Ginda Haro,
Puteri Masri,
Denny Satria
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11s1.26560
Subject(s) - alkaloid , maceration (sewage) , antioxidant , chemistry , dpph , chloroform , extraction (chemistry) , zanthoxylum , traditional medicine , indole alkaloid , ethanol , chromatography , food science , organic chemistry , medicine , materials science , composite material
Objective: Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical-induced oxidative stress. The purpose of this study is to determine antioxidant activities of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits.Methods: Z. acanthopodium DC. fruit was extracted by maceration. Ethanol extract was fractionated with liquid-liquid extraction using n-hexane and chloroform at pH 3, 7, 9, and 11 to obtained alkaloid fractions. Antioxidant activity for extract and fractions was determined with 1,1-diphenyl-2- picrylhydrazyl.Results: The IC50 of extract and fractions was 101.11±0.11, 273.24±0.12, 116.62±0.04, 61.12±0.01, 23.15±0.01, and 112.39±0.04 μg/mL, respectively.Conclusion: The results reveal that alkaloid fractions of Z. acanthopodium DC. fruit have very strong antioxidant potential. Our further study is to isolate the alkaloid compounds.