Open AccessCYTOPROTECTIVE ACTIVITY OF ETHANOL FRACTION OF COLEUS AMBOINICUS LOUR. LEAVES AGAINST VERO CELLS INDUCED BY H2O2
Author(s) -
Reny Haryani,
Urip Harahap,
Masfria Masfria,
Denny Satria
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11s1.26559
Subject(s) - vero cell , viability assay , hydrogen peroxide , mtt assay , coleus , ethanol , chemistry , traditional medicine , apoptosis , flow cytometry , reactive oxygen species , microbiology and biotechnology , pharmacology , biochemistry , biology , botany , medicine , in vitro
Objective: The objective of this study is to assess the cytoprotective activity of Coleus amboinicus Lour. leaves ethanol fraction (EtF) in Vero cells which induced by hydrogen peroxide (H2O2).Methods: Cytoprotective activity was determined using 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) method, apoptosis activity was analyzed by flow cytometry, and expression of radical spesies oxygen (ROS) was observed by imunocytochemistry.Result: The EtF (100 μg/mL) was showed the highest percent viability 74.42±0.28% using MTT method. Furthermore, the EtF was increased viability of Vero cell after induced with 0.8 mM H2O2 and EtF was decreased the expression of ROS.Conclusion: Based on the description, EtF has cytoprotective activity towards Vero cells which induced by 0.8 mM H2O2 .