Open AccessDEVELOPMENT OF POLYHERBAL WITH ANTIOXIDANT ACTIVITY
Author(s) -
Jain Deepak,
Anurekha Jain
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i8.27791
Subject(s) - antioxidant , ascorbic acid , dpph , chemistry , phytochemical , tannin , traditional medicine , glycoside , food science , chromatography , biochemistry , organic chemistry , medicine
Objective: The objective of this research was to develop a polyherbal formulation (PHF) using four different herbs and to evaluate their phytochemicals, physical constants, and determination of their antioxidant activity by DPPH method.Methods: The PHF authenticated herbs were characterized by studying its morphological and pharmacognostic characters. Phytochemical screening showed the presence of alkaloids, glycosides, carbohydrates, amino acid, tannin, steroids, and flavonoids in the combination extract. Physical parameters such as loss on drying (LOD), pH, ash values, LOD, and extractive value have been studied. The antioxidant activity of the combination of extract (100 mg each) was determined using DPPH free radical scavenging method.Results: The results showed that the combination extract has best antioxidant effect at a dose of 400 μg/ml when it was compared with ascorbic acid as the reference standard.Conclusion: The results obtained in this research work clearly showed that the combination extract has best antioxidant effect at a dose of 400 μg/ ml when it was compared with ascorbic acid as the reference standard.