Open AccessDETERMINATION OF NITRAZEPAM IN PHARMACEUTICAL PREPARATIONS USING SOLID-PHASE REACTOR - REVERSE FLOW INJECTION ANALYSIS
Author(s) -
Mariam Jamal,
Hind Hadi
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i7.26644
Subject(s) - nitrazepam , chromatography , reagent , chemistry , particle size , analytical chemistry (journal) , dosage form , calibration curve , linear range , detection limit , materials science , organic chemistry , medicine , diazepam , pharmacology
Objective: A simple and fast reverse flow injection system including a solid-phase reactor containing PbO2 with spectrophotometric detection was suggested for the determination of nitrazepam (NIT) in pharmaceutical tablets.Methods: The method was based on oxidation of the reagent (phloroglucinol) with PbO2 immobilized in a polymeric matrix which was then coupled with reduced NIT in aqueous medium. The pink-colored product was measured at 530 nm.Results: The calibration graph was linear over the range of 50–400 μg/mL with a relative standard deviation of <2% (n=29) and a sample throughput of 48 samples per hour. The variables of the solid-phase reactor such as composition, particle size, and length of the reactor were studied. The chemical and physical parameters, which affect the reverse flow method, were also studied.Conclusion: The oxidation reactor engaged with a flow system was successfully applied for the determination of NIT with good sensitivity and precision.