Open AccessTHE ANTIBACTERIAL EVALUATION OF HALICLONA ASSOCIATED BACTERIA AND THE RELATING COMPOUNDS DERIVED FROM THE HOST
Author(s) -
Tutik Murniasih,
Masteria Yunovilsa Putra,
Tri Aryono Hadi
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i7.25510
Subject(s) - bacteria , 16s ribosomal rna , antibacterial activity , sponge , actinobacteria , strain (injury) , metabolite , chemistry , secondary metabolite , biology , microbiology and biotechnology , chromatography , biochemistry , botany , gene , genetics , anatomy
Objective: The objective of this study was to investigate the active compounds derived from Haliclona sp. associated bacteria collected from Untung Jawa Island, Jakarta.Methods: The bacterial isolation, screening of antibacterial activity, purification, and prediction of potential compounds using liquid chromatography– mass spectroscopy/mass spectroscopy (LC–MS/MS) as well as 16S rDNA bacterial characterization were conducted.Results: The potential extract of bacterial strain UJ.17.10-4 showed the highest anti-Gram-positive bacteria. The 16S rDNA gene of this strain had 99% similarity with the actinobacteria Agromyces tropicus. Chemical separation of supernatant extract yielded 13 potent fractions. Identification of antibacterial compounds contained in active fractions using LC-MS/MS were halistanol sulphate C([M+H]+:703), cyclic bis-1,3- dialkylpyridinium([M+H]+: 213.72). In previous research reported that these compounds were isolated from Haliclona sponge and showed anticancer activity.Conclusion: This result supported the ideas that A. tropicus plays an important role in synthesizing, halistanol sulfate C, haliclorensin, and cyclic bis- 1,3-dialkylpyridinium metabolite derived from the host.